Journal: Communications Biology

Article Title: High-throughput screening for cell binding and repulsion peptides on multifunctionalized surfaces

doi: 10.1038/s42003-024-06541-7

Figure Lengend Snippet: a Design of a peptide array from overlapping peptide fragments of functional proteins. b Incubation well of a tray with a schematically shown region of the peptide array (dashed green line). c Fluorescent image of a peptide array fragment with fluorescently labelled cells. Some peptide pixels exhibit strong cell repulsion (black pixels in the peptide array region). The peptide spot size is 4 × 4 s-pixels (120 µm x 120 µm). d Peptide mapping of a selected region of the frizzled-related protein 1 (SFRP1) after incubation with fluorescently labelled cancer cells. Five replicas representing each peptide were arranged in columns. Movement along overlapping peptides shows characteristic cell deposition with good adherence (left), repulsion (middle), or island-like patterns (right). Scale bar: 120 µm.

Article Snippet: The slide was then transferred to a live cell incubation chamber (PeCon GmbH, Erbach, Germany) (37 °C) and observed for a period of 24 h. Representative pictures and movies were processed using ZEN (Zeiss, Jena, Germany) and iMovie (Apple, Cupertino, CA, USA) softwares.

Techniques: Peptide Microarray, Functional Assay, Incubation